Fabrication of antibody microarrays by light-induced covalent and oriented immobilization.
作者： Avijit K. Adak , Ben-Yuan Li , Li-De Huang , Ting-Wei Lin , Tsung-Che Chang
DOI: 10.1021/AM502011R
关键词:
摘要: Antibody microarrays have important applications for the sensitive detection of biologically important target molecules and as biosensors for clinical applications. Microarrays produced by oriented immobilization of antibodies generally have higher antigen-binding capacities than those in which antibodies are immobilized with random orientations. Here, we present a UV photo-cross-linking approach that utilizes boronic acid to achieve oriented immobilization of an antibody on a surface while retaining the antigen-binding activity of the …
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