Analysis of Pineapple Mealybug Wilt Associated Virus -1 and -2 for Potential RNA Silencing Suppressors and Pathogenicity Factors
作者： Kishore Dey , Wayne Borth , Michael Melzer , Ming-Li Wang , John Hu
DOI: 10.3390/V7030969
关键词:
摘要: Higher plants use RNA silencing to defend against viral infections. As a counter defense, plant viruses have evolved proteins that suppress silencing. Mealybug wilt of pineapple (MWP), an important disease pineapple, has been associated with at least three distinct viruses, Pineapple mealybug virus -1, -2, and -3 (PMWaV-1, -3). Selected open reading frames (ORFs) PMWaV-1 PMWaV-2 were screened for their local systemic suppressor activities in Agrobacterium-mediated transient assays using green fluorescent protein (GFP) Nicotiana benthamiana. Results indicate utilizes multiple-component suppression mechanism. Two proteins, p20 CP, target both N. benthamiana, while the p22 CPd only In related PMWaV-1, we found one encoded p61, had activity. Of all tested from suppressed induced by double-stranded (dsRNA), but when low levels inducing dsRNA used. None analyzed could interfere short distance spread We examined mechanism activity investigating effect PMWaV-2-encoded CP on secondary siRNAs. Our results suggest block signal repressing production also demonstrate enhanced pathogenicity Potato X
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G.P. Martelli, A.A. Agranovsky, M. Bar-Joseph, D. Boscia, T. Candresse, R.H.A. Coutts, V.V. Dolja, B.W. Falk, D. Gonsalves, W. Jelkmann, A.V. Karasev, A. Minafra, S. Namba, H.J. Vetten, G.C. Wisler, N. Yoshikawa, The family Closteroviridae revised Archives of Virology. ,vol. 147, pp. 2039- 2044 ,(2002) , 10.1007/S007050200048
Tamas Dalmay, Rob Horsefield, Thomas Hartig Braunstein, David C Baulcombe, SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis The EMBO Journal. ,vol. 20, pp. 2069- 2078 ,(2001) , 10.1093/EMBOJ/20.8.2069
Paulo Gouveia, Susana Dandlen, Ângela Costa, Natália Marques, Gustavo Nolasco, Identification of an RNA silencing suppressor encoded by Grapevine leafroll-associated virus 3 European Journal of Plant Pathology. ,vol. 133, pp. 237- 245 ,(2012) , 10.1007/S10658-011-9876-1
Valerian V. Dolja, Jan F. Kreuze, Jari P.T. Valkonen, Comparative and functional genomics of closteroviruses. Virus Research. ,vol. 117, pp. 38- 51 ,(2006) , 10.1016/J.VIRUSRES.2006.02.002
Christophe Himber, Patrice Dunoyer, Guillaume Moissiard, Christophe Ritzenthaler, Olivier Voinnet, Transitivity-dependent and -independent cell-to-cell movement of RNA silencing. The EMBO Journal. ,vol. 22, pp. 4523- 4533 ,(2003) , 10.1093/EMBOJ/CDG431
Atsushi Takeda, Kazuhiko Sugiyama, Hideaki Nagano, Masashi Mori, Masanori Kaido, Kazuyuki Mise, Shinya Tsuda, Tetsuro Okuno, Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Letters. ,vol. 532, pp. 75- 79 ,(2002) , 10.1016/S0014-5793(02)03632-3
Paulo Gouveia, Gustavo Nolasco, The p19.7 RNA silencing suppressor from Grapevine leafroll-associated virus 3 shows different levels of activity across phylogenetic groups Virus Genes. ,vol. 45, pp. 333- 339 ,(2012) , 10.1007/S11262-012-0772-3
Jonathan C Reed, Kristin D Kasschau, Alexey I Prokhnevsky, Kodetham Gopinath, Gregory P Pogue, James C Carrington, Valerian V Dolja, Suppressor of RNA silencing encoded by Beet yellows virus Virology. ,vol. 306, pp. 203- 209 ,(2003) , 10.1016/S0042-6822(02)00051-X
Cecilia Sarmiento, Eva Gomez, Merike Meier, Tony A. Kavanagh, Erkki Truve, Cocksfoot mottle virus P1 suppresses RNA silencing in Nicotiana benthamiana and Nicotiana tabacum Virus Research. ,vol. 123, pp. 95- 99 ,(2007) , 10.1016/J.VIRUSRES.2006.07.013
A. Molnar, C. W. Melnyk, A. Bassett, T. J. Hardcastle, R. Dunn, D. C. Baulcombe, Small Silencing RNAs in Plants Are Mobile and Direct Epigenetic Modification in Recipient Cells Science. ,vol. 328, pp. 872- 875 ,(2010) , 10.1126/SCIENCE.1187959