Critical cellulase and hemicellulase activities for hydrolysis of ionic liquid pretreated biomass.
作者： Christopher J. Barr , Jeffrey A. Mertens , Constance A. Schall
DOI: 10.1016/J.BIORTECH.2011.10.101
关键词:
摘要: Critical cellulase and hemicellulase activities are identified for hydrolysis of ionic liquid (IL) pretreated poplar switchgrass; rich substrates with largely amorphous cellulose. Enzymes from Aspergillus nidulans were expressed purified: an endoglucanase (EG) a cellobiohydrolase (CBH), endoxylanase (EX) acetylxylan esterase (AXE). β-Xylosidase (βX) Selenomonas ruminantium commercial β-glucosidase (βG) Novozyme 188 admixed the A. enzymes. Statistical analysis indicates that βG βX significant both glucose xylose yields two substrates. EG is factor glucan while EX xylan The CBH, which has activity on crystalline cellulose negligible cellulose, was not in hydrolysis. poplar. addition AXE significantly improves but switchgrass.
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