An Overview of RNA Sequence Analyses: Structure Prediction, ncRNA Gene Identification, and RNAi Design.

摘要: This unit briefly describes the two fundamentally different methods for predicting RNA structures. The first is to find that structure with minimum free energy of folding, as predicted by various thermodynamic parameters related base-pair stacking, loop lengths, and other features. If one has only a single sequence, this approach best available method. second fundamental prediction use multiple, homologous sequences which can infer common structure, then try predict all sequences. Such an referred comparative method or phylogenetic prediction.