ABE Fermentation From Low Cost Substrates

摘要: The high cost of substrate and product inhibition in the fermentation broth remain two major problems associated with bio-butanol production. This thesis aims to solve these by examining abundant lignocellulosic biomass as potential feedstocks exploring novel substrates such carbohydrates derived from microalgae for ABE (Acetone-butanol-ethanol) fermentation. commonly observed toxic effect after pretreatment lignocelluslosic was removed resin adsorption, where could also serve an in-situ butanol recovery device. Corn cobs (an agricultural waste), switchgrass energy crop) phragmites invasive plant North America) were investigated Clostridium saccharobutylicum DSM 13864. NaOH followed a washing step used reduce recalcitrance facilitate subsequent enzymatic hydrolysis. Total sugar yields corn cobs, 475, 365, 385 g/kg raw biomass, respectively. After fermentation, yield 166, 146, 150 obtained. Although biofuel production is considered more sustainable than food crops, it still faces many challenges. In order demonstrate possible strategy using microalgal lipid extracted (LEA) To convert carbohydrate fraction into solvents (ABE), LEA either acid hydrolysed glucose or directly fermented. highest titers (8.05 g/L) obtained hydrolysates. However fermenting hydrolysate required detoxification via resin, while direct did not, significantly simplifying process. that detoxify hydrolysates further recovery. Detoxifcation hydrolyzed L-493, improved fermentability signficantly. Resin L-493 efficient removing phenolic comopunds present hydrolysates, well produced during