Isolation and characterization of homocholine-degrading Pseudomonas sp. strains A9 and B9b

摘要: Soil isolates, identified as Pseudomonas sp. strain A9 and B9b (based on the phenotypic features phylogenetic analysis) were found to degrade homocholine aerobically. Morphological char- acterization using optical microscope under light phase contrast conditions showed that cells of formed short rods measuring approximately 0.5-1 9 1.5- 2.0 lm in size while those long 2.5-3.0 during early growth both nutrient broth basal-homocholine (basal-HC) media. Strain was able grow basal-HC medium at a wide range temperatures (4-41C) whereas not either 4 or 41C. Comparative 16S rRNA sequencing studies indicated fell into putida subclade located fulva subclade. Washed strains degraded completely within 6 h with concomitant formation several metabolites. Analysis metabolites by capillary electrophoresis, fast atom bombardment-mass spectrometry, gas chromatogra- phy-mass trimethylamine (TMA) major metabolite beside b-alanine betaine tri- methylaminopropionaldehyde. Therefore, possible degradation pathway isolated is through successive oxidation alcohol group (-OH) aldehyde (-CHO) acid (-COOH), thereafter cleavage C-N bonds yielding methylamine an alkyl chain.