Transcriptional Regulation of furA and katG upon Oxidative Stress in Mycobacterium smegmatis

摘要: The DNA region upstream of katG in Mycobacterium smegmatis was cloned and sequenced. furA gene, highly homologous to tuberculosis furA, mapped this region. furA-katG organization appears be conserved among several mycobacteria. transcription pattern M. upon oxidative stress analyzed by Northern blotting primer extension. Although both induced stress, transcripts covering genes could not identified either or reverse transcriptase PCR. Specific 5′ ends were for katG, respectively. By cloning regions a reporter we demonstrated the presence two promoters, pfurA, located immediately pkatG, within terminal part coding sequence. Transcription from pfurA stress. A 23-bp sequence overlapping −35 is mycobacteria streptomycetes might involved controlling activity. lacking region, suggesting cis-acting regulatory role