Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation

摘要: We compared the effects of phorbol 12-myristate 13-acetate (PMA) and thrombin with those nonlytic concentrations reactive oxygen species (ROS) generated by hypoxanthine (HX)-xanthine oxidase (XO) on adhesion properties human umbilical cord vein endothelial cells (HUVEC) to resting polymorphonuclear neutrophils (PMN). PMN adherence HX-XO-treated HUVEC was increased approximately twofold 2.5-fold relative untreated HUVEC, both immediately after 2 hours. It not additive that induced PMA or stimulation HUVEC. ROS-induced due platelet-activating factor (PAF) P-selectin expression, as it neither antagonized BN52021 (PAF receptor antagonist) nor inhibited anti-P-selectin monoclonal antibody (MoAb), contrary PMA- thrombin-stimulated preincubated mannose-6-P N- acetylneuraminic acid (sialic acid), but mannose galactose-6-P, showed reduced ROS-treated suggesting carbohydrate molecules were expressed latter served ligand for L-selectin. Intercellular molecule (ICAM- 1), constitutively present surface involved in since this anti-ICAM-1, anti-CD11a, anti-CD11b, anti-CD18 MoAbs. A non-CD18, non-ICAM-1-dependent mechanism is also adherence, these MoAbs additive; moreover, combinations anti-ICAM-1 sialic completely adherence. The binding HX-XO-exposed observed here IC-AM-1, independent its upregulation, another mechanism, which carbohydrates recognize L-selectin PMN.