Metabolic engineering of Cyanidioschyzon merolae

摘要: Algae are expected to be promising alternative sources of biofuels, foods, and cosmetics. The unicellular red alga Cyanidioschyzon merolae is potentially useful for producing high concentrations desirable biomaterials by metabolic engineering. C. genetically traceable can thrive at low pH (1–5) temperatures (25–50 °C), which harmful many other organisms. Thus, this suitable outdoor cultivation without the risk contamination from undesirable Recent studies regarding have reported enhanced triacylglycerol (TAG) production, used biodiesel genetic modification. Introducing cyanobacterial acyl-acyl carrier protein (ACP) reductase in led temporary TAG accumulation via an artificial pathway. omics analyses showed that acyl-ACP expression resulted upregulating endogenous aldehyde dehydrogenase fatty acid synthetic pathway chloroplasts. Another study expressed 12-kDa FK506-binding Saccharomyces cerevisiae succeeded increasing levels adding rapamycin. suggested target rapamycin (TOR) regulated TAG-synthesizing enzymes, glycerol-3-phosphate acyltransferase, acyl-CoA:diacylglycerol acyltransferase. Therefore, combination engineering evaluation effects will help understanding regulatory mechanism metabolism. In addition, recent started find culture conditions increase while maintaining cellular growth. Combinations these techniques manipulations leads production biomolecules on a large scale future.