Halobacterial S9 operon. Three ribosomal protein genes are cotranscribed with genes encoding a tRNA(Leu), the enolase, and a putative membrane protein in the archaebacterium Haloarcula (Halobacterium) marismortui.
作者： W.J. Krömer , E. Arndt
DOI: 10.1016/S0021-9258(18)54267-8
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摘要: Abstract In the eubacterium Escherichia coli genes for ribosomal proteins L13 and S9 form an operon consisting of two genes. The corresponding archaebacterium Haloarcula marismortui (Halobacterium was recently reassigned to genus [Oren, A., Ginzburg, M., B. Z., Hochstein, L. I., Volcani, E. (1990) Int. J. Syst. Bacteriol. 40, 209-210] is now called marismortui) which presented here, much larger encoding three (HL29, HmaL13, HmaS9), a tRNA(Leu), glycolytic enzyme enolase (HmaEno), putative membrane protein (OrfMSG), not yet identified open reading frames (OrfMMV, OrfMNA). nucleotide sequence 3931 base pairs has been established. Northern analysis revealed existence polycistronic mRNA (3.7 kilobases) demonstrating that transcription gene pathway coupled Upstream first (tRNA(Leu)) promoter structure typical extreme halophilic archaebacteria detected downstream last (OrfMSG) terminator present. As shown by S1-nuclease mapping, tRNA-mRNA transcript as well alone present in vivo. transcriptional start point cleavage are be almost identical 5' 3' ends, respectively, mature tRNA. C-terminal part OrfMSG shows significant similarity vertebrate laminin receptor protein.
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