Temporal Changes in Allocation and Partitioning of New Carbon as 11C Elicited by Simulated Herbivory Suggest that Roots Shape Aboveground Responses in Arabidopsis

摘要: Using the short-lived isotope 11C (t1/2 = 20.4 min) as 11CO2, we captured temporal changes in whole-plant carbon movement and partitioning of recently fixed into primary secondary metabolites a time course (2, 6, 24 h) following simulated herbivory with well-known defense elicitor methyl jasmonate (MeJA) to young leaves Arabidopsis (Arabidopsis thaliana). Both 11CO2 fixation 11C-photosynthate export from labeled source leaf increased rapidly (2 MeJA treatment relative controls, preferential allocation radiolabeled resources belowground. At same time, remaining aboveground sink tissues showed MeJA-treated, leaves, where it was incorporated 11C-cinnamic acid. By h, resource toward roots returned control levels, while increased. This corresponded an increase invertase activity accumulation phenolic compounds, particularly anthocyanins, leaves. Induction phenolics suppressed sucrose transporter mutant plants (suc2-1), indicating that this phenomenon may be controlled, part, by phloem loading at However, when plant were chilled 5°C disrupt flow between above- belowground tissues, failed allocate or damaged wounding suggesting play integral role controlling how respond defensively aboveground.