Industrial yellow wine yeast haploid separation method

摘要: The invention discloses an industrial yellow wine yeast haploid separation method. In the method, HO gene knockout component is formed by a Cre/loxp and G418 resistance maker KanMX gene, successfully converted into yeast, of knocked out homologous recombination, consequentially, strain separated, meanwhile, galactose culture medium induces expression Cre recombinant enzyme so as to cut off kanMX that without external obtained. method can be used for quickly efficiently separating has advantages high rate survival rate, simple technology low production cost, lays material base subsequent metabolic engineering modification genetic research on yeast.