Phospholipase C-β1 is a GTPase-activating protein for Gq/11, its physiologic regulator
作者： Gabriel Berstein , Jonathan L. Blank , Deok-Young Jhon , John H. Exton , Sue Goo Rhee
DOI: 10.1016/0092-8674(92)90165-9
关键词:
摘要: Abstract Purified M1 muscarinic cholinergic receptor and G q/11 were coreconstituted in lipid vesicles. Addition of purified phospholipase C-β1 (PLC-β1) further stimulated the receptor-promoted steady-state GTPase activity up to 20-fold. Stimulation depended upon receptor-mediated GTP-GDP exchange. PLC-β1 caused a rapid burst hydrolysis -bound GTP that was at least 50-fold faster than its absence. Thus, stimulates acts as GTPase-activating protein (GAP) for physiologic regulator, . GTPase-stimulating specific both Such GAP by an effector coupled trimeric can reconcile slow pure proteins vitro with fast deactivation protein-mediated signaling.
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sci-hub.se 参考文章(45)
T. Higashijima, J. Burnier, E.M. Ross, Regulation of Gi and Go by mastoparan, related amphiphilic peptides, and hydrophobic amines. Mechanism and structural determinants of activity. Journal of Biological Chemistry. ,vol. 265, pp. 14176- 14186 ,(1990) , 10.1016/S0021-9258(18)77284-0
P G Suh, S H Ryu, W C Choi, K Y Lee, S G Rhee, Monoclonal antibodies to three phospholipase C isozymes from bovine brain. Journal of Biological Chemistry. ,vol. 263, pp. 14497- 14504 ,(1988) , 10.1016/S0021-9258(18)68247-X
K Haga, T Haga, Purification of the muscarinic acetylcholine receptor from porcine brain. Journal of Biological Chemistry. ,vol. 260, pp. 7927- 7935 ,(1985) , 10.1016/S0021-9258(17)39541-8
P.C. Sternweis, J.K. Northup, M.D. Smigel, A.G. Gilman, The regulatory component of adenylate cyclase. Purification and properties. Journal of Biological Chemistry. ,vol. 256, pp. 11517- 11526 ,(1981) , 10.1016/S0021-9258(19)68431-0
E M Parker, K Kameyama, T Higashijima, E M Ross, Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells Journal of Biological Chemistry. ,vol. 266, pp. 519- 527 ,(1991) , 10.1016/S0021-9258(18)52467-4
G Berstein, J.L. Blank, A.V. Smrcka, T Higashijima, P.C. Sternweis, J.H. Exton, E.M. Ross, Reconstitution of agonist-stimulated phosphatidylinositol 4,5-bisphosphate hydrolysis using purified m1 muscarinic receptor, Gq/11, and phospholipase C-beta 1. Journal of Biological Chemistry. ,vol. 267, pp. 8081- 8088 ,(1992) , 10.1016/S0021-9258(18)42410-6
D R Brandt, E M Ross, GTPase activity of the stimulatory GTP-binding regulatory protein of adenylate cyclase, Gs. Accumulation and turnover of enzyme-nucleotide intermediates. Journal of Biological Chemistry. ,vol. 260, pp. 266- 272 ,(1985) , 10.1016/S0021-9258(18)89726-5
T Higashijima, K M Ferguson, M D Smigel, A G Gilman, The effect of GTP and Mg2+ on the GTPase activity and the fluorescent properties of Go. Journal of Biological Chemistry. ,vol. 262, pp. 757- 761 ,(1987) , 10.1016/S0021-9258(19)75850-5
V A Florio, P C Sternweis, Mechanisms of muscarinic receptor action on Go in reconstituted phospholipid vesicles. Journal of Biological Chemistry. ,vol. 264, pp. 3909- 3915 ,(1989) , 10.1016/S0021-9258(19)84939-6
S E Navon, B K Fung, Characterization of transducin from bovine retinal rod outer segments. Mechanism and effects of cholera toxin-catalyzed ADP-ribosylation. Journal of Biological Chemistry. ,vol. 259, pp. 6686- 6693 ,(1984) , 10.1016/S0021-9258(20)82197-8