Bone marrow derived mesenchymal stem cells inhibit the proliferative and profibrotic phenotype of hypertrophic scar fibroblasts and keloid fibroblasts through paracrine signaling.

摘要: Abstract Background Hypertrophic scars and keloids, characterized by over-proliferation of fibroblasts aberrant formation the extracellular matrix (ECM), are considered fibrotic diseases. Accumulating evidence indicates that mesenchymal stem cells (MSCs) promote scar-free wound healing inhibit tissue formation, making them a potentially effective therapeutic treatment for hypertrophic keloids. Objective To investigate paracrine effects bone marrow derived MSCs (BMSCs) on biological behavior scar (HSFs) keloid (KFs). Methods Proliferative profibrotic phenotype changes were analyzed immunofluorescence staining, in-cell western blot, real-time PCR. Results BMSC-conditioned medium inhibited HSF KF proliferation migration, but did not induce apoptosis. Interestingly, normal skin fibroblast-conditioned exhibited no inhibitory or migration. Furthermore, significantly decreased expression genes, including connective growth factor, plasminogen activator inhibitor-1, transforming factor-β 1 , 2 in HSFs KFs at both transcriptional translational levels. In contrast, antifibrotic such as 3 decorin, was substantially enhanced under same culture conditions. Finally, we observed suppressed ECM synthesis KFs, indicated collagen I fibronectin low levels hydroxyproline cell supernatant. Conclusion These findings suggest BMSCs attenuate proliferative associated with through signaling mechanism.