The mammalian ecdysoneless protein interacts with RNA helicase DDX39A to regulate nuclear mRNA export.

摘要: The mammalian orthologue of Ecdysoneless (ECD) protein is required for embryogenesis, cell cycle progression, and mitigation ER stress. Here, we identified key components the mRNA export complexes as binding partners ECD characterized functional interaction with export-related DEAD BOX helicase DDX39A. We find that involved in RNA through its knockdown (KD) blocks from nucleus to cytoplasm, which rescued by expression full length but not mutant defective have previously shown overexpressed ErbB2+ breast cancers (BC). In this study, extended analyses two publically available BC TCGA METABRIC data sets. both dataset, overexpression correlated short patient survival, specifically BC. dataset also poor survival TNBC . Further, KD ErbB2+BC cells led a decrease ErbB2 level due block nuclear export, was associated impairment oncogenic traits. These findings provide novel mechanistic insight into physiological pathological function ECD.