Establishment and Characterization of a Singaporean Chinese Lung Adenocarcinoma Cell Line with Four Copies of the Epidermal Growth Factor Receptor Gene
作者： Meng Ling Choong , Jacklyn Yong , Yu Wang , May Ann Lee
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摘要: Abstract We have established a lung adenocarcinoma cell line, ETCC016, from pleural effusion of male Singaporean Chinese with advanced adenocarcinoma. The subject smoked 20 cigarettes per day for more than 30 years. line arose spontaneous transformation cells grown in collagen-coated culture dish. Transformed characteristics the include ability to reach high confluency dish, low doubling time, form colonies soft agar, and solid tumor immune-compromised SCID mice. Immunostaining showed that originated epithelial cells. Genomic analysis revealed large amount chromosomal aberrations (gain loss genetic materials, heterozygosity [LOH]), indicative long history smoking. four copies epidermal growth factor receptor (EGFR) three MYC, but lost one copy RB1 gene. LOH was detected TP53 BRAF genes. There is no anaplast...
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