Biacore analysis with stabilized G-protein-coupled receptors.
作者： Rebecca L. Rich , James Errey , Fiona Marshall , David G. Myszka
关键词:
摘要: Using stabilized forms of β1 adrenergic and A2A adenosine G-protein-coupled receptors, we applied Biacore to monitor receptor activity characterize binding constants small-molecule antagonists spanning >20,000 fold in affinity. We also illustrate an improved method for tethering His-tagged receptors on NTA chips yield stable, high-capacity, high-activity surfaces, as well a novel approach regenerate receptor-binding sites. Based our success with this approach, expect that the combination biosensor technology will become common characterizing members family.
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sci-hub.se 参考文章(23)
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