Functional interactions between the fusion protein and hemagglutinin-neuraminidase of human parainfluenza viruses.

摘要: The fusion glycoprotein (F) and hemagglutinin-neuraminidase (HN) genes of human parainfluenza virus type 2 (PI2) were molecularly cloned expressed in HeLa-T4 cells by using the vaccinia virus-T7 transient expression system. Expression F HN proteins was detected immunoprecipitation surface immunofluorescence staining. Although protein found to be cleaved into F1 F2 on cell surfaces, no observed. However, cotransfection F-protein gene together with P12 resulted significant levels fusion. Cell also observed when separate cultures transfected F-expressing mixed HN-expressing cells. Surprisingly, PI2 3 (PI3) protein, detectable Similarly, upon coexpression PI3 proteins. extensive fusion, which resembled result. These results indicate that under conditions used, is unable cause itself provides a specific function cannot provided another paramyxovirus attachment protein. Further, suggest type-specific functional interaction between involved mediating