Impact of alkylphospholipids on the gene expression profile of HaCaT cells.

摘要: Objective New alkylphospholipids (APLs) that are structurally derived from the platelet-activating factor (PAF) promising candidates for anticancer treatment. After incorporation into cell membranes, APLs able to interfere with a wide variety of key enzymes implicated in growth, motility, invasion, and apoptosis. In addition prototype 1-O-octadecyl-2-O-methyl-rac-glycero-3- phosphocholine (edelfosine), we presented novel group APLs, glycosidated phospholipids efficiently inhibit proliferation. Two members this group, Ino-C2-PAF Glc-PAF, display high efficacy low cytotoxicity immortalized nontumorigenic skin keratinocyte line, HaCaT. This study investigated impact on transcription whole genome. Materials methods Using Agilent complementary DNA microarray technology, compared global gene expression profiles HaCaT cells treated edelfosine, Ino-C2-PAF, or Glc-PAF profile control cells. Results We found has strongest influence comparison edelfosine Glc-PAF. Gene Ontology analysis showed differentially expressed transcripts regulated by three mainly lipid metabolism, biosynthesis, differentiation, development, ion homeostasis. Nevertheless, most remarkable finding is represented ability downregulate broad spectrum genes associated regulation innate acquired immune response linked inflammation. Conclusion These results identify as effective APL used study. Therefore, might be compound further studies concentrate inhibition inflammatory responses.