Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice

摘要: Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence. The aim was to establish validate local distant spread of subcutaneously previously injected fluorescence transduced human tongue cell lines epithelial mesenchymal phenotype nude mice. A total 32 four-week-old male athymic Balb/c mice randomly allocated into 4 groups ( ). single dose 0.3 mL PBS containing 1 × 107 four different cell-lines (UM1, UM1-GFP, UM2, UM2-RFP) the right side their posterolateral back. Validity assessment cells’ tumorigenicity assessed by physical examination, imaging, histology weeks after injection. tumor take rate similar animals either parental or cells. Transduced easily detectable vivo cryosection using fluorescent imaging. UM1 showed increased mean volume, presenting disorganized histopathological patterns. Fluorescence do not change injection vivo.