Transcriptomic, Functional, and Network Analyses Reveal Novel Genes Involved in the Interaction Between Caenorhabditis elegans and Stenotrophomonas maltophilia.

摘要: The bacterivorous nematode Caenorhabditis elegans is an excellent model for the study of innate immune responses to a variety bacterial pathogens, including emerging nosocomial pathogen Stenotrophomonas maltophilia. this interaction has ecological and medical relevance as S. maltophilia found in association with C. other nematodes wild opportunistic pathogen. We identified 393 genes that were differentially expressed when exposed virulent avirulent strains strain E. coli. then used probabilistic functional gene network (WormNet) determine 118 formed interacting set highly connected eight or more predicted interactions. hypothesized these might play important role defense against maltophila mutations six seven have significant effect on survival response bacteria. Of genes, C48B4.1, mpk-2, cpr-4, clec-67, lys-6 are needed combating JCMS strain, while dod-22 was solely involved K279a strain. further clec-67 up regulated vs. K279a, down regulated. Only had documented immunity, which demonstrates merit our approach identification novel infection.