Tumor necrosis factor, macrophage colony-stimulating factor, and interleukin 1 production within sponge matrix allografts.

摘要: Neither the presence nor specific role of secretory cytokines in vivo allograft rejection has been extensively studied. We quantitated levels colony-stimulating factors, tumor necrosis factor, and interleukin 1 within rejecting allograft. BALB/c (H-2d) mice were implanted with polyurethane sponges containing either allogeneic C57BL/6 (H-2b) or syngeneic splenocytes, splenocyte-free media. At various days postgrafting, harvested, cells infiltrating grafts analyzed for antidonor cytolytic activity, while IL-1, TNF, CSF measured graft exudate fluid. Allogeneic had significantly higher concentrations CSF, IL-1 than grafts. A radioimmunoassay revealed that macrophage factor (M-CSF) is primary produced Peak TNF preceded peak M-CSF levels, which coincided initial appearance allospecific cytotoxic T lymphocytes. Maximal CTL activity was seen on day 13, when these already begun to fall. Specific bioassays multi-CSF (IL-3), granulocyte granulocyte-macrophage IL-2, IL-4 failed detect sponge fluid at any time. hypothesize M-CSF, probably play regulatory roles immunologic events site challenge.