Phosphatidylinositol 3-kinase activity is important for progesterone-induced Xenopus oocyte maturation.

摘要: Abstract In somatic cells, phosphatidylinositol 3-kinase (PI3 kinase) is a critical intermediary in growth factor-induced mitogenesis. We have examined the role of this enzyme meiotic maturation Xenopus laevis oocytes. PI3 kinase activity was present immunoprecipitates p85 subunit from immature oocytes and markedly increased following progesterone stimulation. Injection bacterially expressed protein corresponding to C-terminal SH2 domain (SH2-C) inhibited progesterone-induced activation maturation. N-terminal or SH3 did not inhibit SH2-C oocyte induced by c-mos RNA injection. In addition, radiolabelled used probe lysates, revealing that novel 200-kDa bound SH2-C. This may be an important mediator lipid metabolism