Regulation of interleukin-8 by miR-17 during chronic inflammation in cystic fibrosis

摘要: A symptom of cystic fibrosis (CF) is chronic lung inflammation. MicroRNA (miRNA) profiles between CF bronchial epithelial cells (BECs) differ from that non-CF BECs. 1 Several studies, including this one, reveal functional roles miRNAs in and immunology. Aims: To establish a causal relationship miR-17 downregulation CF. identify targets relevant to pathophysiology. Methods: In vivo miRNA expression was analysed human mouse tissue. gene with qPCR ELISA. Potential were identified silico . Further studies target validation mimics/inhibitors luciferase reporter assays conducted inflammatory models Results: MiR-17 downregulated airway tissue compared samples. This confirmed tracheal CF-like β-ENaC-transgenic model, but not CFTR knockout BEC cultures exposed 5-day stimulation resulted miR-17, no base-line difference found CFTR- CFTR+ cell lines. Target analysis revealed interleukin-8 (IL-8). IL-8 subsequently validated as cultures. Conclusion: We show an important the immune system development, BECs subjected disease conditions. could have major role controlling inflammation through regulation IL-8. 1. Oglesby et al. miR-126 regulates TOM1 J Immunol 184, 1702–1709 (2010).