Measurement of the Antiplatelet Effects of Aspirin in Cerebrovascular Disease

摘要: To the Editor: We read with interest recent article on use of PFA-100 in measuring ex vivo response to aspirin therapy patients cerebrovascular disease.1 Most were assessed acute phase after ischemic stroke or transient attack, although some asymptomatic stenosis an extracranial intracranial artery also studied. Thirty seven per cent overall “aspirin resistant” using PFA-100, and rate resistance was higher receiving 81 mg (56%) than those 325 daily (28%). However there are a number issues that warrant further discussion. The authors report “PFA-100 device uses flow cytometry paradigm determine ability whole blood close aperture stimulation collagen epinephrine.”1 However, it must be emphasized readers significant differences between techniques methodology used test system. Flow is method for sensing cells, including platelets, as they liquid stream through laser beam.2 Platelets either blood, platelet-rich plasma, separation from plasma (washed platelets),3 may injected cell, aligned single file transparent nonfluorescent “sheath fluid” exerts pressure cell suspension being When platelet passes beam, light scattered different directions platelets can identified by their scatter characteristics. The sample labeled fluorochrome-linked monoclonal antibodies specific certain antigens surface, stained fluorescent dyes. degree fluorescence subsequently quantify expression activation marker interest, …