Characterization of NADH: nitrate reductase from the coccolithophorid Emiliania huxleyi (Lohman) Hay & Mohler (Haptophyceae).
作者： Koji Iwamoto , Yoshihiro Shiraiwa
DOI: 10.1007/S10126-002-0051-8
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摘要: Abstract Nitrate reductase was purified from and characterized in a bloom-forming unicellular calcifying alga, Emiliania huxleyi (Haptophyceae). The molecular masses of the native form subunit were 514 85 kDa, respectively, showing that enzyme is hexamer composed 6 homologous subunits. K m values for NADH NO3− 40 µM 104 µM, respectively. Activity reduction nitrate very high with reduced methylviologen NADH, but no activity observed NADPH or flavin mononucleotide; oxidation cytochrome c did not occur ferricyanide. These results indicate NADH-specific (EC 1.6.6.1), among algae plants its structure kinetic properties are unique.
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