Essential Oil from Bark of Aniba parviflora (Meisn.) Mez (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model.
作者： Felipe P de Oliveira , Ana Carolina B da C Rodrigues , Emilly JSP de Lima , Valdenizia R Silva , Luciano de S Santos
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摘要: Aniba parviflora (Meisn.) Mez (Lauraceae) is an aromatic plant of the Amazon rainforest, which has a tremendous commercial value in perfumery industry; it popularly used as flavoring sachets and baths. In Brazilian folk medicine, A. to treat victims snakebites. Herein, we analyzed chemical composition bark essential oil (EO) its effect on growth human hepatocellular carcinoma HepG2 cells in vitro in vivo. EO was obtained by hydrodistillation characterized GC-MS GC-FID. The main constituents were linalool (16.3±3.15), α-humulene (14.5±2.41 %), δ-cadinene (10.2±1.09 %), α-copaene (9.51±1.12 %) germacrene B (7.58±2.15 %). Initially, EO's cytotoxic evaluated against five cancer cell lines (HepG2, MCF-7, HCT116, HL-60 B16-F10) one non-cancerous (MRC-5), using Alamar blue method after 72 h treatment. calculated IC50 values 9.05, 22.04, >50, 15.36, 17.57, 30.46 μg/mL, respectively. best selectivity for with selective index 3.4. DNA Fragmentation cycle distribution quantified flow cytometry treatment period 24 48 h. tumor development in vivo xenograft model C.B-17 SCID mice engrafted cells. vivo inhibition at doses 40 80 mg/kg 12.1 62.4 %,
fiocruz.br参考文章(30)
Ana Prata, Clarissa Rocha, Emmanoel Costa, Daniel Bezerra, Ana Rodrigues, Larissa Bomfim, Sara Neves, Leociley Menezes, Rosane Dias, Milena Soares, Antitumor Properties of the Essential Oil From the Leaves of Duguetia gardneriana Planta Medica. ,vol. 81, pp. 798- 803 ,(2015) , 10.1055/S-0035-1546130
A.M. Bittencourt, O.R. Gottlieb, W.B. Mors, M.Taveira Magalhães, S. Mageswaran, W.D. Ollis, I.O. Sutherland, The natural occurrence of 6-styryl-2-pyrones and their synthesis Tetrahedron. ,vol. 27, pp. 1043- 1048 ,(1971) , 10.1016/S0040-4020(01)92506-2
I. Nicoletti, G. Migliorati, M.C. Pagliacci, F. Grignani, C. Riccardi, A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry Journal of Immunological Methods. ,vol. 139, pp. 271- 279 ,(1991) , 10.1016/0022-1759(91)90198-O
Sandra S Ribeiro, Aline M de Jesus, Charlene S dos Anjos, Thanany B da Silva, Alan DC Santos, Jemmyson R de Jesus, Moacir S Andrade, Tais S Sampaio, Wesley F Gomes, Péricles B Alves, Adriana A Carvalho, Claudia Pessoa, Manoel O de Moraes, Maria LB Pinheiro, Ana Paula N Prata, Arie F Blank, Renata Silva-Mann, Valeria RS Moraes, Emmanoel V Costa, Paulo Cesar L Nogueira, Daniel P Bezerra, None, Evaluation of the cytotoxic activity of some Brazilian medicinal plants. Planta Medica. ,vol. 78, pp. 1601- 1606 ,(2012) , 10.1055/S-0032-1315043
Jérémie Sœur, Laurent Marrot, Philippe Perez, Ismail Iraqui, Guy Kienda, Michèle Dardalhon, Jean-Roch Meunier, Dietrich Averbeck, Meng-Er Huang, None, Selective cytotoxicity of Aniba rosaeodora essential oil towards epidermoid cancer cells through induction of apoptosis. Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 718, pp. 24- 32 ,(2011) , 10.1016/J.MRGENTOX.2010.10.009
Rosana P.C. Ferraz, Diogo S. Bomfim, Nanashara C. Carvalho, Milena B.P. Soares, Thanany B. da Silva, Wedna J. Machado, Ana Paula N. Prata, Emmanoel V. Costa, Valéria Regina S. Moraes, Paulo Cesar L. Nogueira, Daniel P. Bezerra, Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae) Phytomedicine. ,vol. 20, pp. 615- 621 ,(2013) , 10.1016/J.PHYMED.2013.01.015
S. Ansar Ahmed, Robert M. Gogal, Jane E. Walsh, A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay Journal of Immunological Methods. ,vol. 170, pp. 211- 224 ,(1994) , 10.1016/0022-1759(94)90396-4
C.M.Andrade da Mata Rezende, M.V. von Bülow, O.R. Gottlieb, S.Lamêgo Vieira Pinho, A.I. da Rocha, The 2-pyrones of Aniba species Phytochemistry. ,vol. 10, pp. 3167- 3172 ,(1971) , 10.1016/S0031-9422(00)97370-9
R. S. Galaverna, P. T. B. Sampaio, L. E. S. Barata, M. N. Eberlin, C. H. V. Fidelis, Differentiation of two morphologically similar Amazonian Aniba species by mass spectrometry leaf fingerprinting Analytical Methods. ,vol. 7, pp. 1984- 1990 ,(2015) , 10.1039/C4AY02598A
H. van Den Dool, P. Dec. Kratz, A GENERALIZATION OF THE RETENTION INDEX SYSTEM INCLUDING LINEAR TEMPERATURE PROGRAMMED GAS-LIQUID PARTITION CHROMATOGRAPHY. Journal of Chromatography A. ,vol. 11, pp. 463- 471 ,(1963) , 10.1016/S0021-9673(01)80947-X