Molecular Indicators Used in the Development of Predictive Models for Microbial Source Tracking

摘要: A number of chemical, microbial, and eukaryotic indicators have been proposed as fecal pollution sources in water bodies. No single one the tested to date has able determine source water. However, combined use different demonstrated be best way defining predictive models suitable for determining sources. Molecular methods are promising tools that could complement standard microbiological analysis. In this study, feasibility some molecular microbial tracking (MST) was compared (names markers parentheses): host-specific Bacteroidetes (HF134, HF183, CF128, CF193), Bifidobacterium adolescentis (ADO), dentium (DEN), gene esp Enterococcus faecium, mitochondrial DNA associated with humans, cattle, pigs (Humito, Bomito, Pomito, respectively). None individual enabled 100% identification. They should other raise sensitivity specificity increase identified. MST using only these were developed. The evaluated by considering lowest needed obtain highest rate identification three (ADO, Pomito) correct 75.7% samples, differentiation between human, swine, bovine, poultry Discrimination human nonhuman possible two markers: ADO Pomito (84.6% identification). percentage increased analyzed. model distinguishing from based on 5 ADO, DEN, provided 90.1% classification.