Exotoxin A of Pseudomonas aeruginosa: active, cloned toxin is secreted into the periplasmic space of Escherichia coli.

摘要: We subcloned the structural gene for exotoxin A (ETA) of Pseudomonas aeruginosa in front tac promoter an Escherichia coli expression vector and studied intracellular location properties protein product. The E. K-12 strain that carried this recombinant plasmid produced immunoreactive was identical to authentic ETA size cytotoxic ADP-ribosyl transferase activities per unit material. predominantly periplasmic fraction; a mutation secA blocked secretion, processing, conversion fully toxic conformation. results indicate yields native ETA, which is localized within space. This organism may therefore serve as useful host studying structure function ETA. Images