Induction of Cell Death by Betulinic Acid through Induction of Apoptosis and Inhibition of Autophagic Flux in Microglia BV-2 Cells.
作者： Jeongbin Seo , Juneyoung Jung , Dae Sik Jang , Joungmok Kim , Jeong Hee Kim
DOI: 10.4062/BIOMOLTHER.2016.255
关键词:
摘要: Betulinic acid (BA), a natural pentacyclic triterpene found in many medicinal plants is known to have various biological activity including tumor suppression and anti-inflammatory effects. In this study, the cell-death induction effect of BA was investigated BV-2 microglia cells. cytotoxic cells with IC₅₀ approximately 2.0 µM. Treatment resulted dose-dependent chromosomal DNA degradation, suggesting that these underwent apoptosis. Flow cytometric analysis further confirmed BA-treated showed hypodiploid content. treatment triggered apoptosis by decreasing Bcl-2 levels, activation capase-3 protease cleavage PARP. addition, induced accumulation p62 increase conversion LC3-I LC3-II, which are important autophagic flux monitoring markers. The LC3-II indicates autophagosome formation, however, represents downstream autophagy pathway blocked. It demonstrated cell death inducing inhibiting flux. These data may provide new information towards understanding mechanisms induce
sci-hub.st 参考文章(49)
Isei Tanida, Takashi Ueno, Eiki Kominami, LC3 and Autophagy. Methods of Molecular Biology. ,vol. 445, pp. 77- 88 ,(2008) , 10.1007/978-1-59745-157-4_4
Christopher B. McLeland, Jamie Rodriguez, Stephan T. Stern, Autophagy monitoring assay: qualitative analysis of MAP LC3-I to II conversion by immunoblot. Methods of Molecular Biology. ,vol. 697, pp. 199- 206 ,(2011) , 10.1007/978-1-60327-198-1_21
Eric H. Baehrecke, Autophagy: dual roles in life and death? Nature Reviews Molecular Cell Biology. ,vol. 6, pp. 505- 510 ,(2005) , 10.1038/NRM1666
Keisuke Mochida, Yu Oikawa, Yayoi Kimura, Hiromi Kirisako, Hisashi Hirano, Yoshinori Ohsumi, Hitoshi Nakatogawa, Receptor-mediated selective autophagy degrades the endoplasmic reticulum and the nucleus Nature. ,vol. 522, pp. 359- 362 ,(2015) , 10.1038/NATURE14506
Jie Liu, Dongping Huang, Jing Xu, Jiabin Tong, Zishan Wang, Li Huang, Yufang Yang, Xiaochen Bai, Pan Wang, Haiyun Suo, Yuanyuan Ma, Mei Yu, Jian Fei, Fang Huang, Tiagabine Protects Dopaminergic Neurons against Neurotoxins by Inhibiting Microglial Activation Scientific Reports. ,vol. 5, pp. 15720- 15720 ,(2015) , 10.1038/SREP15720
R. G. Morris, A. H. Wyllie, M. J. Arends, Apoptosis. The role of the endonuclease American Journal of Pathology. ,vol. 136, pp. 593- 608 ,(1990)
Noboru Mizushima, Tamotsu Yoshimori, How to Interpret LC3 Immunoblotting Autophagy. ,vol. 3, pp. 542- 545 ,(2007) , 10.4161/AUTO.4600
Kaoru Saijo, Christopher K. Glass, Microglial cell origin and phenotypes in health and disease Nature Reviews Immunology. ,vol. 11, pp. 775- 787 ,(2011) , 10.1038/NRI3086
P Vogel, E Dux, C Wiessner, Evidence of apoptosis in primary neuronal cultures after heat shock Brain Research. ,vol. 764, pp. 205- 213 ,(1997) , 10.1016/S0006-8993(97)00458-7
Subhadip Mukhopadhyay, Prashanta Kumar Panda, Niharika Sinha, Durgesh Nandini Das, Sujit Kumar Bhutia, Autophagy and apoptosis: where do they meet? Apoptosis. ,vol. 19, pp. 555- 566 ,(2014) , 10.1007/S10495-014-0967-2