Binding of 4-methylumbelliferyl beta-D-galactopyranoside to Momordica charantia lectin: fluorescence-quenching studies.
作者： M. Islam KHAN , Tripti MAZUMDER , Debkumar PAIN , Namita GAUR , Avadhesha SUROLIA
DOI: 10.1111/J.1432-1033.1981.TB05087.X
关键词:
摘要: The binding of 4-methylumbelliferyl beta-D-galactopyranoside (MeUmb-Galp), to Mormordica charantia lectin was studied by equilibrium dialysis and quenching ligand fluorescence. fluorescence MeUmb-Galp decreases as a function solvent polarity. On M. lectin, its nearly 100% quenched, showing that the glycoside takes place in hydrophobic environment. fluorescent sugar saccharide-specific evidenced reversal lactose. association constant is independent experimental method used at 25 degrees C value (1.96 +/- 0.05) X 10(4) M-1. number sites determined agree very well with each other; n being equal 1.98 0.02. Ka for also competition studies employing ka obtained lactose 1.21 M-1 30 C. internal consistency site values low high saturation indicates absence additional subsite on lectin. thermodynamic parameters do not differ greatly change temperature; - delta H S are 9.63 kJ mol-1 21 0.3 J K-1 respectively range 15--35 indicting saccharide exothermic nature.
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