Differentiation of Human Embryonic Stem Cells into Neural Lineage Cells

摘要: Human embryonic stem cells (hESCs) derived from preimplantation embryos have unique properties of unlimited self-renewal and pluripotent development potential. Therefore, their derivatives can not only serve as an source for the use in transplantation therapy pharmaceutical screens, but also provide vitro models to further our understanding both normal abnormal human development. For neural differentiation hESCs, various protocols available so far generate different functional neurons glia, offering opportunities study early induction specification. In this chapter, we will begin with brief introduction principles, then overview three major approaches used most often currently, including cell aggregation, stromal coculture adherent monolayer induction. The discussion hESC-based be included. Finally, challenges possible solutions discussed.