Error-Prone Processing of Apurinic/Apyrimidinic (AP) Sites by PolX Underlies a Novel Mechanism That Promotes Adaptive Mutagenesis in Bacillus subtilis

摘要: In growing cells, apurinic/apyrimidinic (AP) sites generated spontaneously or resulting from the enzymatic elimination of oxidized bases must be processed by AP endonucleases before they compromise cell integrity. Here, we investigated how and processing these noncoding lesions Nfo, ExoA, Nth contribute to production mutations (hisC952, metB5, leuC427) in starved cells Bacillus subtilis YB955 strain. Interestingly, this strain that were deficient for accumulated a greater amount stationary phase than during exponential growth. Moreover, under growth-limiting conditions, triple nfo exoA nth knockout significantly increased amounts adaptive his, met, leu revertants produced B. parental Of note, number stationary-phase-associated reversions alleles was decreased following disruption polX. contrast, growth, reversion rates three tested polymerase X (PolX). Therefore, postulate can through novel mechanism mediated error-prone PolX DNA polymerase.