Replication Study: Wnt activity defines colon cancer stem cells and is regulated by the microenvironment.

摘要: As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Evans et al., 2015), that described how intended to replicate selected experiments from paper 'Wnt activity defines colon cancer stem cells and is regulated by microenvironment' (Vermeulen 2010). Here, report results. Using three independent primary spheroidal cultures expressed Wnt reporter construct observed high was associated with cell surface markers CD133, CD166, CD29, but not CD24 CD44, while original study found all five were correlated (Figure 2F; Vermeulen Clonogenicity highest in clonogenic potential low increased myofibroblast-secreted factors, including HGF. While effects same direction as 6D; 2010) whether statistical significance reached among different conditions varied. When tested vivo, did find difference tumorigenicity between activity, more effective inducing tumors 7E; Tumorigenicity, however, which 2010), statistically significant. Finally, meta-analyses for each results where possible.