Isolation of Murine Adult Bone Marrow and Fetal Liver Cells for Mechanistic Assessment of Hematotoxicity Caused by Organic Solvents

摘要: Isolation of adult bone marrow and fetal liver cells from murine models is an important technique that can be a valuable tool to study mechanisms hematotoxicity. Organic solvents are class industrial chemicals target the leading hematatoxicity including leukemia. Many these bioactivated into reactive intermediates, which initiate oxidative stress; thus, identification quantification stress essential gain further understanding organic solvent-initiated toxicity. This chapter describes procedure for isolating mice cells, primary site hematopoiesis in developing embryo. Additionally, descriptions molecular techniques employed identify quantify caused by exposure included.