COTTON OVULE CULTURE: A TOOL FOR BASIC BIOLOGY, BIOTECHNOLOGY AND COTTON IMPROVEMENT

摘要: Nearly 30 years ago the conditions for culturing immature cotton ovules were established to serve as a working research tool investigating physiology and biochemistry of fiber development. Not only has this tissue culture method been employed characterize plant cell expansion secondary wall synthesis, but ovule cultures have contributed numerous other aspects development well. In addition basic studies on development, used examine plant-fungal interactions, model low temperature stress responses, elucidate pathways responsible pigment formation in naturally pigmented probe how cytoskeletal elements regulate organization. Success rescuing Gossypium interspecific hybrids was dependent media formulations that could support early embryo ovulo. As tissues produced are analyzed by increasingly more sophisticated techniques, there appear be some differences between growth planta vitro. Discerning is different from field-grown plants can contribute valuable information crop improvement. Cotton an especially attractive system studying effects gravity elongation, cellulose biosynthesis excellent targets examining transient expression introduced gene constructs. With minor modification, procedure originally described C. A. Beasley I. P. Ting growing vitro will continue useful foreseeable future.