Amyloid β-protein (Aβ) associated with lipid molecules: immunoreactivity distinct from that of soluble Aβ
作者： Katsuhiko Yanagisawa , JoAnne McLaurin , Makoto Michikawa , Avijit Chakrabartty , Yasuo Ihara
DOI: 10.1016/S0014-5793(97)01484-1
关键词:
摘要: We previously identified a novel amyloid β-protein (Aβ), that binds to GM1 ganglioside, in brains exhibiting the early pathological changes of AD. In this study, we raised monoclonal antibodies, using membrane fractions containing abundant ganglioside-bound Aβ as antigens. Monoclonal antibody 4396, produced immunoprecipitates Aβ42 with diffuse plaques, but does not react soluble or ganglioside. Furthermore, recognizes bound lipid vesicles and unexpectedly, phosphatidylinositol. contrast, control anti-Aβ recognize these vesicles. These results indicate associated lipids has an immunoreactivity distinct from A.
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