Analysis of a brain-specific isozyme. Expression and chromatin structure of the rat aldolase C gene and transgenes.

摘要: Abstract Aldolase C mRNA is detected by Northern blot in all fetal tissues rat; it very abundant the adult brain and undetectable other tissues. However, reverse transcriptase polymerase chain reaction amplification indicates that this gene not totally repressed these A DNase-I hypersensitivity site located a 115-base pair proximal promoter fragment detectable as well Two MspI/HpaII restriction sites at -3800 -450 base pairs are demethylated or partially methylated In transgenic mice, 12.5-kilobase genomic strongly tissue specifically expressed different lines, with conservation of methylation pattern similar to endogenous gene. chloramphenicol acetyltransferase directed either 800 115 aldolase 5'-flanking sequences but level expression low. This greatly increased when transgene consists hybrid 5.5 kilobases sequences. We propose therefore chromatin structure around accessible tissues, then remains open brain, where active, which practically inactive. The specificity conferred short needs more upstream be fully active.