Detection and Quantification of Spongospora subterranea in Soil, Water and Plant Tissue Samples Using Real-Time PCR
作者： Pieter van de Graaf , Alison K. Lees , Danny W. Cullen , James M. Duncan
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摘要: A sensitive real-time polymerase chain reaction (PCR) assay was developed for the quantification of Spongospora subterranea, cause powdery scab and root galling in potato, vector Potato mop top virus. specific primer pair a fluorogenic TaqMan® probe were designed to perform quantitative detection S. subterranea soil, water plant tissue samples. The tested using DNA from cystosori, zoospores, plasmodia zoosporangia pathogen. extracted directly cystosori suspended clay soil with varying levels added cystosori. obtained zoospores released into nutrient solution by presence tomato bait plants also tested, as infected roots. In many cases, successful even at low inoculum levels. This could therefore be useful tool studying biology optimisation disease avoidance control measures.
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