Analyses of movement and contact of two nucleated cells using a gas-driven micropipette aspiration technique.

摘要: Adhesion between two nucleated cells undergoes specific significances in immune responses and tumor metastasis since cellular adhesive molecules usually express on apposed cell membranes. However, quantification of the interactions is still challenging microvasculature. Here distinct systems were used, including three types human (Jurkat or PMN vs. MDA-MB-231 cell) kinds murine native (PMN liver sinusoidal endothelial cell). Cell movement, compression to, relaxation from counterpart quantified using an in-house developed gas-driven micropipette aspiration technique (GDMAT). This assay robust to quantify this process movement contact inside a pipette are independent repeated test cycles. Measured approaching retraction velocity follows well normal distribution, which cycle period. Contact area duration also fits Gaussian distribution moreover linearly correlated with positively related gas flux but negatively associated medium viscosity. adhesion tends reach equilibrium state increase period duration. These results further understanding dynamics