The C proteins of heterogeneous nuclear ribonucleoprotein complexes interact with RNA sequences downstream of polyadenylation cleavage sites.

摘要: The heterogeneous nuclear ribonucleoprotein C1 and C2 proteins were preferentially cross-linked by treatment with UV light in extracts to RNAs containing six different polyadenylation signals. domain required for the interaction was located downstream of poly(A) cleavage site, since deletion this segment from several substrate greatly reduced cross-linking efficiency. In addition, only sequences efficiently C proteins, while fully processed, polyadenylated not. Analysis mutated variants simian virus 40 late signal showed that uridylate-rich region between 30 55 nucleotides site efficient proteins. This addition has been shown influence efficiency reaction. However, there not a strict correlation polyadenylation.