Efficient, specific, developmentally appropriate cre-mediated recombination in anterior pituitary gonadotropes and thyrotropes

摘要: Summary Tissue-specific expression of cre recombinase is a well-established genetic tool to analyze gene function, and it limited only by the efficiency specificity available mouse strains. Here, we report generation transgenic line containing cassette with codon usage optimized for mammalian cells (iCre) under control glycoprotein hormone α-subunit (αGSU) regulatory sequences in bacterial artificial chromosome genomic clone. Initial analysis this line, Tg(αGSU-iCre), reporter strains reveals onset activity differentiating developing anterior pituitary gland at embryonic day 12.5, pattern characteristic endogenous αGSU. In adult mice, αGSU-iCre was active lobe that produce αGSU (gonadotropes thyrotropes) high penetrance. Little or no observed other tissues, including skeletal cardiac muscle, brain, kidney, lungs, testis, ovary, liver. This suitable efficient, specific, developmentally regulated deletion floxed alleles gonadotropes thyrotropes. genesis 51:785–792. © 2013 Wiley Periodicals, Inc.