Effects of Adolescent Intermittent Alcohol Exposure on the Expression of Endocannabinoid Signaling-Related Proteins in the Spleen of Young Adult Rats.

摘要: Intermittent alcohol exposure is a common pattern of consumption among adolescents and known to modulate the expression endocannabinoid system (ECS), which involved in metabolism inflammation. However, it unknown whether this may have short-term consequences on ECS spleen. To address question, we examined plasma concentrations metabolic inflammatory signals splenic early adult rats exposed during adolescence. A 4-day drinking dark (DID) procedure for 4 weeks was used as model intermittent forced-alcohol administration (20%, v/v) female male Wistar rats, were sacrificed 2 after last DID session. First, there no liver damage or alterations parameters. certain altered according sex exposition. Whereas fractalkine [chemokine (C-X3-C motif) ligand 1] only affected by with lower concentration an interaction between TNF-α interleukin-6 displayed changes. Regarding mRNA protein ECS, receptors endocannabinoid-synthesizing enzymes found be area-specific patterns Overall, whereas cannabinoid receptor CB1 nuclear peroxisome proliferator-activated PPARα alcohol-exposed compared control CB2 higher. Additionally, N-acyl-phosphatidylethanolamine-specific phospholipase D high low rats. In conclusion, adolescence sufficient induce changes signaling-related proteins pro-inflammatory cytokines young strong sexual dimorphism. The potential impact these adulthood remains elucidated.