Transient expression of lacZ in particle bombarded Gracilaria changii (Gracilariales, Rhodophyta)
作者： Sook-Yee Gan , Song Qin , Rofina Yasmin Othman , Daozhan Yu , Siew-Moi Phang
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摘要: Using a Biolistic PDS 1000/He system, healthy thalli of Gracilaria changii were bombarded with gold particles coated plasmid DNA containing the lacZ reporter gene. Transient expression was observed in under rupture-disc pressures 4482, 6206, 7584 and 8963 KPa, two days after bombardment. Although G. exhibits slight blue background, positive background colour can be clearly differentiated. The results indicate that could useful gene SV40 promoter an effective for transformation.
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