Dynamic distribution and the role of abscisic acid during seed development of a lady's slipper orchid, Cypripedium formosanum.

摘要: Background and Aims Although abscisic acid (ABA) is commonly recognized as a primary cause of seed dormancy, there lack information on the role ABA during orchid development. In order to address this issue, localization quantification were determined in developing seeds Cypripedium formosanum. Methods The endogenous profile was measured by enzyme-linked immunosorbent assay (ELISA). Temporal spatial distributions visualized immunohistochemical staining with monoclonal antibodies. Fluoridone applied test causal relationship between content germinability. Key Results low at proembryo stage, then increased rapidly from 120 150 days after pollination (DAP), accompanied progressive decrease water germination. Immunofluorescence signals indicated an increase fluorescence over time stage maturation. From immunogold labelling, gold particles could be seen within cytoplasm embryo-proper cells early stages As approached maturity, observed periplasmic space, plasmalemma cells, surface wall embryo proper, inner walls seed-coat cells. At found mainly apoplast, such shrivelled outer coats. Injection fluoridone into capsules resulted enhanced germination mature seeds. Conclusions results indicate that key inhibitor C. formosanum. distinct accumulation pattern suggests it synthesized cytosol development, exported apoplastic region for subsequent regulatory processes approach maturity.