Generation and Characterisation of a Reference Transcriptome for Phalaris (Phalaris aquatica L.)
作者： Rebecca C Baillie , Michelle C Drayton , Luke W Pembleton , Sukhjiwan Kaur , Richard A Culvenor
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摘要: Phalaris aquatica is a cool-season perennial grass species that extensively cultivated in Australia, with additional usage other areas of the world. displays number desirable agronomic characteristics, although unfavourable traits include excessive seed shattering, sensitivity to aluminium toxicity, and several toxicosis syndromes. Varietal development has date been based on traditional selection methods, but would benefit from application genomics-based approaches, which require large-scale sequence resources. Due large nuclear DNA content, methods target expressed component genome reduce complexity analysis are most amenable current sequencing technologies. A reference unigene set developed by transcriptome multiple tissues single plant belonging variety Landmaster. Comparisons have made gene complements related species, as well protein databases, patterns expression different evaluated. candidate genes relevant removal undesirable attributes identified. The will provide basis for detailed studies differential identification potential transgenic deployment, critical resource genotypic support future genomics-assisted breeding activities phalaris improvement.
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