DNA aptamers selectively target Leishmania infantum H2A protein.

摘要: Parasites of the genus Leishmania produce leishmaniasis which affects millions people around world. Understanding molecular characteristics parasite can increase knowledge about mechanisms underlying disease development and progression. Thus, study features histones has been considered particular interest because does not condense chromatin during mitosis and, consequently, a different role for these proteins in biology be expected. Furthermore, sequence divergences amino carboxy-terminal domains kinetoplastid core convert them potential diagnostic and/or therapeutics targets. Aptamers are oligonucleotide ligands that selected vitro by their affinity specificity target as consequence tertiary structure they able to acquire depending on sequence. Development high-affinity molecules with ability recognize specifically is essential progress this kind study. Two aptamers infantum H2A histone were cloned from previously obtained ssDNA enriched population. These sequenced subjected an silico analysis. ELONA, slot blot Western performed establish aptamer LiH2A ELONA assays using peptides corresponding overlapped sequences made mapping aptamers:LiH2A interaction. As “proofs concept”, used determine number parasites platform purify complex mixtures. The showed secondary structures among them; however, both same located side protein. In addition, we demonstrate useful identification also may application system laboratory tool purification purpose.