Sensitivity of the response of cytosolic calcium in Quin-2-loaded rat hepatocytes to glucagon, adenine nucleosides, and adenine nucleotides.
作者： F D Sistare , R A Picking , R C Haynes
DOI: 10.1016/S0021-9258(17)38939-1
关键词:
摘要: Hepatocytes from fasted rats were used to study the effect of glucagon on intracellular free cytosolic Ca2+ ([Ca2+]i) indicated by use Quin-2-calcium fluorescence. It was found that, in both male and female rats, increased [Ca2+]i at a half-maximally effective concentration (Kact) 0.3 nM, known be half-maximal for affecting several hepatic functions. Acute chelation extracellular did not obliterate hormone but shortened its duration. Cyclic AMP, 5'-AMP, ADP, ATP also [Ca2+]i, while adenosine 2':3'-monophosphate 3'-AMP not. The rise brought about near physiological concentrations may responsible stimulation glutamate metabolism produced acutely glucagon.
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