Selective two-photon collagen crosslinking in situ measured by Brillouin microscopy
作者： Sheldon J. J. Kwok , Ivan A. Kuznetsov , Moonseok Kim , Myunghwan Choi , Giuliano Scarcelli
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摘要: Two-photon polymerization has enabled the precise microfabrication of three-dimensional structures with applications spanning from photonic microdevices, drug delivery systems, and cellular scaffolds. We present two-photon collagen crosslinking (2P-CXL) intact corneal tissue using riboflavin femtosecond laser irradiation. The fiber orientations photobleaching were characterized by second harmonic generation fluorescence imaging, respectively. measurement local changes in longitudinal mechanical moduli confocal Brillouin microscopy visualization cross-linked pattern without perturbation surrounding non-irradiated regions. 2P-CXL-induced stiffening was comparable to that achieved conventional one-photon CXL. Our results demonstrate ability selectively stiffen biological situ at high spatial resolution, broad implications ophthalmology, surgery, engineering.
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