Decreasing lactate level and increasing polypeptide production by downregulating ldh and pdhk expression
作者： 에이미 셴 , 메익시아 자우 , 치 킨 도밍고스 엔지 , 브래들리 리차드 스네데코어
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摘要: The present invention provides methods and compositions for reducing lactate production increasing polypeptide in cultured cells. In one aspect, the relates to a method of culturing cells expressing siRNA specific a) small interfering RNA (siRNA) dehydrogenase (LDH) b) pyruvate kinase (PDHK) comprising steps of: another or vectors an LDH PDHK.
lens.org 参考文章(1)
Brian D. Follstad, Improvement of cell culture performance by inhibitors of pyruvate dehydrogenase kinase ,(2001)